A reliable and sensitive isocratic stability indicating RP-UPLC method has been developed and validated for Quantitative analysis and Content Uniformity study of Terazosin Hydrochloride Dihydrate in tablets. An isocratic method for analysis of Terazosin Hydrochloride Dihydrate was archived on Acquity UPLC BEH C18 (100*2.1) mm particle size 1.7 µ columns within shorter runtime of 6 min with a flow rate of 0.250 ml/min and using a photodiode array detector to monitor the eluent at 246 nm. The mobile phase consisted of Buffer-Acetonitrile (80:20 v/v), (Buffer: 20 mM ortho phosphoric acid in 1L water). The drug was subjected to oxidation, hydrolysis, photolysis and thermal degradation. Response was a liner function of drug concentration in the range of 20-80 µg/ml (r2= 0.9999). Accuracy (recovery) was between 98.21 to 101.60%. Degradation products resulting from the stress studies did not interfere with the detection of Terazosin Hydrochloride Dihydrate and the assay is stability-indicating.
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